Principles of PCR

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Principles of PCRMUPGRET WorkshopJune 18, 2004PCRPolymerase Chain Reaction PCRInitially reagents in…
Principles of PCRMUPGRET WorkshopJune 18, 2004PCR
  • Polymerase Chain Reaction
  • Basic PCR
  • Initially reagents in excess, template and product don’t renature and outcompete primer binding, so amplification occurs at an exponential rate.
  • Timepoint when exponential turns to linear rate is different depending on the initial concentration of the target molecule.
  • Basic PCR
  • To properly quantify one product relative to another you need to be in the exponential phase of amplification for both and know when the end point occurs.
  • Troubleshooting PCR
  • Electrophoresis
  • A way to separate DNA molecules.
  • Related Search
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