Principles of PCR

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Principles of PCRMUPGRET WorkshopJune 18, 2004PCRPolymerase Chain Reactionhttp://www.dnai.org/b/index.htmlhttp://palou.uib.es/roolpi/PCR/Basic PCRInitially reagents in…
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Principles of PCRMUPGRET WorkshopJune 18, 2004PCR
  • Polymerase Chain Reaction
  • http://www.dnai.org/b/index.html
  • http://palou.uib.es/roolpi/PCR/
  • Basic PCR
  • Initially reagents in excess, template and product don’t renature and outcompete primer binding, so amplification occurs at an exponential rate.
  • Timepoint when exponential turns to linear rate is different depending on the initial concentration of the target molecule.
  • Basic PCR
  • To properly quantify one product relative to another you need to be in the exponential phase of amplification for both and know when the end point occurs.
  • Troubleshooting PCR
  • http://www.promega.com/guides/pcr_guide/070_22/promega.html
  • http://info.med.yale.edu/genetics/ward/tavi/Trblesht.html
  • http://palou.uib.es/roolpi/PCR/
  • Electrophoresis
  • A way to separate DNA molecules.
  • http://gslc.genetics.utah.edu/units/biotech/gel/
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