Annona Muricata Leaves Induce G Cell Cycle Arrest and Apoptosis Through Mitochondria-mediated Pathway in Human HCT-116 and HT-29 Colon Cancer Cells

of 2

Please download to get full document.

View again

All materials on our website are shared by users. If you have any questions about copyright issues, please report us to resolve them. We are always happy to assist you.
2 pages
0 downs
anona muricata
  Annona muricata leaves induce G cell cycle arrest and apoptosisthrough mitochondria-mediated pathway in human HCT-116 andHT-29 colon cancer cells I.Product / Ingredient Studieda. Annona muricata L. (Annona muricata) as a member of theAnnonaceae family, the custard apple family, is a small tropical treewidely cultiated throughout the tropical countries. Annona speciessuch as Annona s!uamosa and Annona reticulata are well nown tohae traditional application in the tropicsb.#he popular fruit tree of Annona muricata nown also as $graiola% or$soursop% has e&tensie ethnobotonical uses, such as sedatie,astringent,piscicide,insecticide,ermifuge,hypotensie,antiparasiticandantispasmodic.c.'urthermore, it is traditionally used for the treatment of feers,asthma, pain, coughs, wound and s in remediesd.Annona muricata was described as $the cancer iller% due to itsremar able cytoto&icity against arious cancer cell lines. #he seedsand leaes of Annona muricata hae been widely used by the natiesin South America for the treatment of cancer and tumors.II.Indication a. #o establish the possible apoptotic pathways induced in #*+ and#-- colon cancer cells by ethyl acetate e&tract of Annonamuricata leaes.III.#ype of laima. #he stems, bar s and leaes of Annona muricata reealed noteworthyantiproliferatie eects against cancer cells without aecting normalcellsI0.1osage and Administrationa. #he antiproliferatie potential of the three isolated e&tracts wase&pressed as I23 alue, the concentration of plant e&tract that caused234 inhibition of cell growth which was calculated based on thepercentage of cell iability. As ethyl acetate e&tract of Annona muricataleaes (55A6) showed the strongest I23, which was used to continuethe study.0.#ype of Study (uman or Animal)a.uman cells b.178- (normal human colon epithelial cells)c.#*+ and #-- (human colon cancer cells)0I.Study 1esign (5&perimental or 9bserational)a. 5&perimental in itrob.178- (normal human colon epithelial cells), #*+ and #--(human colon cancer cells) were obtained from the American #ype ellollection (A#, 6anassas, 0A, :SA). #he cells were maintained in;P6I-83 medium (Sigma, St. Louis, 69, :SA) supplemented with  -34 '<S (PAA Lab, Pasching, Austria), 23 =g/ml amphotericin < (PAALab) and -33 :/ml penicillin (PAA Lab) in a humidi>ed atmosphere with24 9 in the air at ?@ -. ells in the e&ponential growth phase werecollected for the following e&periments. #he negatie control for all theassays was represented by the untreated medium containing ehicle16S9 (3.-4).0II.Study Populationa. olon cancer 0III.1uration of the Studya. Incubated for @* h.I.Study 5nd Pointsa. ell iability was ealuated by using the ?(8,2dimethylthiaBol*yl)*,2diphenyltetraBolium (6##) assay. b.In brief, cells (2 & -3 8 cells/ml) were treated with three isolatede&tracts at dierent concentrations in +well plate and incubated for@* h..5thics ommittee Approala. Cot mentioned within the article.I.Limitation of the Studya. Cot stated within the article.II.Study ;esultsa. 55A6 e&erted signi>cant cytoto&ic eects on #-- and #*+ cellsas determined by 6## and L1 assays. b.After *8 h treatment, 55A6 e&hibited the I alue of --.8?@-.7@ mg/mland 7.+7@-.*8 mg/ml against #*+ and #-- cells, respectiely.'low cytometric analysis demonstrated the cell cycle arrest at D- 23phase and phosphatidylserine e&ternaliBation con>rming the inductionof apoptosis. c.55A6 treatment caused e&cessie accumulation of ;9S followed bydisruption of 66P, cytochrome c lea age and actiation of the initiatorand e&ecutioner caspases in both colon cancer cells.ImmunoEuorescence analysis depicted the upregulation of <a& anddownregulation of <cl* proteins while treated with 55A6.d.'urthermore, 55A6 conspicuously bloc ed the migration and inasionof #*+ and #-- cells.III.Source of 5idenceF a. 6oghadamtousi, S.G., et al., Annona muricata leaes induce D- cellcycle arrest and apoptosis through mitochondriamediated pathway inhuman.... Hournal of 5thnopharmacology (*3-8),httpF//d&/.ep.*3-8.37.3--i
Related Search
We Need Your Support
Thank you for visiting our website and your interest in our free products and services. We are nonprofit website to share and download documents. To the running of this website, we need your help to support us.

Thanks to everyone for your continued support.

No, Thanks