West Nile Virus 2002 Laboratory Perspectives Laboratories Branch MOHLTC

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West Nile Virus 2002 Laboratory Perspectives Laboratories Branch MOHLTC. Pauline George. Overview. Test procedures WNV Statistics Laboratory Challenges. Principle of HI Test. Viral Antigen + Goose RBCs = Agglutination
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West Nile Virus 2002Laboratory PerspectivesLaboratories Branch MOHLTC Pauline George Overview Test procedures WNV Statistics Laboratory Challenges Principle of HI Test Viral Antigen + Goose RBCs = Agglutination Serum (Abs) + VAg + GRBCs = No Agglut/Reactive Serum ( no Abs) + VAg + GRBCs = Agglut/Nonreactive Test Procedures-Single Serum HI Test Process
  • Day 1 Extraction
  • Day 2 Absorption
  • Day 2Test Screen/Titrate
  • Day 3 Add RBCs
  • Day 3 Read
  • Confirmatory TestWinnipeg Federal Lab
  • 2 week TAT
  • Plaque Reduction Neutralization test Testing on paired serum against likely viruses Presence/absence neutralizing antibody
  • PCR Tissue/CSF
  • WNV Tests 1999-2002 WNV Tests May - Oct1999-2002 Confirmed West Nile Human Cases - Ontario (to Nov 4, 2002) Probable* West Nile Human Cases - Ontario (to Nov 4, 2002) WVN CasesSummary (to Nov 4, 2002)
  • Confirmed * (PRNT) 49
  • Did not confirm *(PRNT) 9
  • Probable * (HI) 89
  • Suspect ^176 * seroconversion ^ seropositive
  • Laboratory Challenges 2002
  • Substantially increased test volumes
  • Staffing resources
  • Reagent Availability
  • Equipment
  • Antigenic Variation
  • Increased incoming/outgoing telephone calls
  • Other routine tests
  • Training
  • TDG
  • Space Allocation
  • Future Challenges 2002 and Beyond
  • Funding
  • Staffing
  • In-house tests vs Commercial Tests Availability?
  • Case criteria
  • Data Analysis
  • Training
  • Confirmatory Test Availability
  • Antigen consistency
  • Antigen source/cost?
  • Future Challenges2002 and Beyond cont’d
  • For confirmatory testing in Ontario
  • Requires Level 3 Biosafety lab
  • PRNT confirmatory assay
  • PCR
  • Culture
  • Acknowledgements OPHL Arboviruses & Rickettsiae Laboratory Staff Dr. M. Fearon, I Guglielmi, E Cheung, Canadian Science Centre for Human & Animal Health , Winnipeg Viral Zoonotic Staff Dr. M. Drebot
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