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     WHO/HTM/NTD/WHOPES/2009.2 GUIDELINES FOR EFFICACY TESTING OF INSECTICIDES FOR INDOOR AND OUTDOOR GROUND-APPLIED SPACE SPRAY APPLICATIONS CONTROL OF NEGLECTED TROPICAL DISEASES WHO PESTICIDE EVALUATION SCHEME    © World Health Organization 2009  All rights reserved. The designations employed and the presentation of the material in this publication do not imply the expression of any opinion whatsoever on the part of the World Health Organization concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. Dotted lines on maps represent approximate border lines for which there may not yet be full agreement. The mention of specific companies or of certain manufacturers’ products does not imply that they are endorsed or recommended by the World Health Organization in preference to others of a similar nature that are not mentioned. Errors and omissions excepted, the names of proprietary products are distinguished by initial capital letters.  All reasonable precautions have been taken by the World Health Organization to verify the information contained in this publication. However, the published material is being distributed without warranty of any kind, either express or implied. The responsibility for the interpretation and use of the material lies with the reader. In no event shall the World Health Organization be liable for damages arising from its use.  iii CONTENTS Page  ACKNOWLEDGEMENTS V 1. INTRODUCTION 2. LABORATORY STUDIES 1 Intrinsic insecticidal activity 2 Insecticidal activity of active ingredient(s) used as space spray 3 Diagnostic concentration for monitoring resistance 5 Cross-resistance to other active Ingredients 7 3. SMALL-SCALE STUDIES 9 Outdoor applications 10 Indoor applications 13 4. OPERATIONAL TRIALS 14 Outdoor trials 16 Indoor trials 19 5. REFERENCES 21 ANNEX 1. GUIDELINES FOR DEVELOPMENT OF INFORMED CONSENT FORM 22 ANNEX 2. EXAMPLE PRINTOUT OF A COMPUTERIZED PROBIT ANALYSIS 26 ANNEX 3. WIND TUNNEL SPECIFICATIONS 27 ANNEX 4. WHO TUBES AND PROCEDURES FOR TESTING THE SUSCEPTIBILITY OF ADULT MOSQUITOES TO INSECTICIDES 34
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