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You design lecture. Discuss the research that “proved” Rotational Catalysis Review Metabolism and Electron Transport Chain from previous two classes Explain how Glycolysis is like eating Pizza! Talk about Fermentation as an option in some cells
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You design lecture
  • Discuss the research that “proved” Rotational Catalysis
  • Review Metabolism and Electron Transport Chain from previous two classes
  • Explain how Glycolysis is like eating Pizza!
  • Talk about Fermentation as an option in some cells
  • Discuss Krebs Cycle and the path inside the mitochondria like the events in the Biovisions movie
  • Masasuke Yoshida “Ummm… check this out.”Masasuke Yoshida “Ummm… check this out.”Masasuke Yoshida “Ummm… check this out.”Hiroyasu Itoh –designed this approach (Nature 2004)“Reporter enzyme” emits light when detects new ATPAnalyze the data. Given that the gray bars represent the basal/background level of ATP in the experiment, explain what you believe would be (i) predictions v. (ii) observationsFigure 1 Itoh et al, Nature 2004You design lectureProteinsFatsCarbohydratesAminoacidsSugarsGlycerolFattyacidsGlycolysisGlucoseGlyceraldehyde-3-PNH3PyruvateAcetyl CoACitricacidcycleOxidativephosphorylationHomeostasis: What is negative feedback?Inter-mediateInter-mediateStart ofpathwayProductXEnzyme 1Enzyme 3Enzyme 2Presence of product inhibits enzyme 1GlucoseAMPGlycolysisFructose-6-phosphateStimulates+Phosphofructokinase––Fructose-1,6-bisphosphateInhibitsInhibitsPyruvateATPCitrateAcetyl CoACitricacidcycleOxidativephosphorylationThe electron transport chain occurs in the inner membrane of the mitochondrion (in regions called “cristae”)ELECTRON TRANSPORT CHAINH+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+H+QH+H+H+H+Cyt cH+H+H+QH+H+H+Inner membraneH+H+NADHFADH2O2H+NAD+FADH+H+H2OFo unitATPComplex IComplex IIComplex III Complex IVStalkH+F1 unitATP ADP + PiYou design lectureCellular respirationIf electron acceptor (such as oxygen)is presentELECTRON TRANSPORT AND OXIDATIVE PHOSPHORYLATIONKREBS CYCLEGLYCOLYSISGlucose PyruvateIf electron acceptor (such as oxygen) is NOT presentFERMENTATIONAll 10 reactions of glycolysis occur in cytosolGLYCOLYSISWhat goes in:ATPATP12345Glucose-6-phosphateFructose-6-phosphateFructose-1,6-bisphosphateGlucose What comes out:ADPADPGlycolysis begins with an energy-investment phase of 2 ATPPIP
  • Think of the beginning of “Glyco-lysis” as eating a pizza
  • Think Glucose = PizzaThe common ‘hexo-pyranose’ form of Pizza Meet PIP (Phosphorylate-Isomerize-Phosphorylate)
  • Is that really the best way to hold on to your pizza?
  • Aldolase =Fructose 1-6 Bisphosphate -> DHAP (ick) and G-3-P (tasty!)Isomerase ->DHAP (ick) G-3-P (tasty!)Isomerase ->G-3-P (tasty!) G-3-P (tasty!)PIP[ATP] highGLYCOLYSISEnergy-investment phaseWhat goes in:ATPATP12345Glucose-6-phosphateFructose-6-phosphateFructose-1,6-bisphosphateGlucose What comes out:ADPADPHexokinase (capture)phosphoglucoisomerasePFK*??????PFK is highly regulatedATP at active siteFructose- 1,6-bisphosphate at active siteATP at regulatory site[ATP] highGlyceraldehyde-3-phosphate (G3P)-dehydrogenaseGLYCOLYSISEnergy-investment phasealdolaseWhat goes in:DHAPATPATPPIP12345isomeraseGlucose-6-phosphateFructose-6-phosphateFructose-1,6-bisphosphateGlucose What comes out:ADPADPG-3-PhexokinasephosphoglucoisomerasePFK*G-3-Pinhibitsstimulates[ADP] [AMP]Phosphoglycero(PG)-kinaseGlyceraldehyde-3-phosphate(G3P)-dehydrogenasePIPAll 10 reactions of glycolysis occur in cytosolGLYCOLYSISWhat goes in:ATPATP12345Glucose-6-phosphateFructose-6-phosphateFructose-1,6-bisphosphateGlucose What comes out:ADPADPG-3-PG-3-PGlyceraldehyde-3-phosphate(G3P)-dehydrogenaseGlycolysis begins with an energy-investment phase of 2 ATPPhosphoglycero(PG)-kinase2 G-3-Ppyruvate kinaseG-3-P-dehydrogenaseEnergy payoff phaseThe “2” indicates that glucose has been split into two 3-carbon sugars2 NAD+2 ADP2 ADP222226 7 8 9 10Pyruvate1,3-BisPGA3-PGA2 NADH2 ATP2 ATPDuring the energy payoff phase, 4 ATP are produced for a net gain of 2 ATPYou design lectureYou design lectureWhere do we go next?SUMMARY OF GLUCOSE OXIDATION2 NADH 2 Pyruvate Glucose2 ATPMitochondrionCytoplasmPyruvate Dehydrogenase Multienzyme ComplexIf you have O2, enter the MatrixSUMMARY OF GLUCOSE OXIDATION2 NADH 2 Pyruvate Glucose2 ATPMitochondrionCytoplasmWhere do we go next?SUMMARY OF GLUCOSE OXIDATION2 NADH 2 NADH 2 Pyruvate Glucose2 Acetyl CoA 2 CO22 ATPMitochondrionCytoplasmSUMMARY OF GLUCOSE OXIDATION6 NADH2 NADH 2 NADH 2 FADH2KREBS CYCLE2 Pyruvate Glucose2 Acetyl CoA 4 CO22 CO22 ATP2 ATPMitochondrionCytoplasmIs this what the Citric Acid Cycle looks like?Is this what the Citric Acid Cycle really looks like?What about this?Actually…You design lecture
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